•  About:
  Web application programming interface (WAPI) of SProtP Human:
  Usage:
    Please submit your fasta sequences data (should named as "fstc") to "http://reprod.njmu.edu.cn/sprotp/api.py" (action value) in post method. Example result: WAPI of SProtP Human - Example
  Example code:
 •  Submission:
*Today work files: 0; Max work files: 500, Submission count: 1369.
Paste sequence(s) in FASTA format into the field below ( Examples ):
! Note:
1.Please submit no more than 10 sequence(s) at a time. The length of each sequence must be range from 10 to 5000.
2.Non-proteinogenic amino acids will be omit!
3.The program may take some time!
4.Your work file will be automatically deleted after 24 hours!
Output format:
 
 •  Contact:

  To contact us via e-mail, any questions, comments or suggestions are welcome!
     XJG: guo_xuejiang@njmu.edu.cn
     XFS: xfsong@nuaa.edu.cn
  Thank you!

 •  Author:
   Xiaofeng Song1, Tao Zhou2, Hao Jia1, Xuejiang Guo2,*, Xiaobai Zhang1, Ping Han3* and Jiahao Sha2
     1: Department of Biomedical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016 China;
     2: Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, China;
     3: The First Hospital affiliated to Nanjing Medical University, Nanjing 210029 China;
     *: Co-corresponding author
     *Please cite: Song X, Zhou T, Jia H, Guo X, Zhang X, Han P, Sha J. SProtP: a web server to recognize those short-lived proteins based on sequence-derived features in human cells. PLoS One. 2011;6(11):e27836.
 •  Quick Tools:

   Predict transmembrane helices using TMHMM AND Predict the existence of a signal peptide using signalp


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 • FASTA format description:
  A sequence in FASTA format begins with a single-line description, followed by lines of sequence data. The description line is distinguished from the sequence data by a greater-than (">") symbol in the first column. It is recommended that all lines of text be shorter than 80 characters in length. An example sequence in FASTA format is:
>example optional comment
MAPWAEAEHSALNPLRAVWLTLTAAFLLTLLLQLLPPGLLPGCAIFQDLIRYGKT KCGEPSRPAACRAFDVPKRYFSHFYIISVLWNGFLLWCLTQSLFLGAPFPSWLHG
LLRILGAAQFQGGELALSAFLVLVFLWLHSLRRLFECLYVSVFSNVMIHVVQYCF
GLVYYVLVGLTVLSQVPMDGRNAYITGKNLLMQARWFHILGMMMFIWSSAHQYKC
HVILGNLRKNKAGVVIHCNHRIPFGDWFEYVSSPNYLAELMIYVSMAVTFGFHNL
TWWLVVTNVFFNQALSAFLSHQFYKSKFVSYPKHRKAFLPFLF

 
 • Standard amino acid abbreviations:
  1.Proteinogenic amino acids:[ARNDCEQGHILKMFPSTWYV]
  2.Non-proteinogenic amino acids:[UOBJZX]
    2.1 Additional amino acids:[UO]
    2.2 Ambiguous Amino Acids:[BJZX]
  *For SProtP, the accepted amino acid codes are Proteinogenic amino acids, note that Non-proteinogenic amino acids will be omit!
Amino Acid 3-Letter 1-Letter
Alanine Ala A
Arginine Arg R
Asparagine Asn N
Aspartic acid Asp D
Cysteine Cys C
Glutamic acid Glu E
Glutamine Gln Q
Glycine Gly G
Histidine His H
Isoleucine Ile I
Leucine Leu L
Lysine Lys K
Methionine Met M
Phenylalanine Phe F
Proline Pro P
Serine Ser S
Threonine Thr T
Tryptophan Trp W
Tyrosine Tyr Y
Valine Val V
Selenocysteine Sec U
Pyrrolysine Pyl O
Asparagine or aspartic acid Asx B
Glutamine or glutamic acid Glx Z
Leucine or Isoleucine Xle J
Unspecified or unknown amino acid Xaa X

 


 • Sequences Examples:
>example_1
MAPWAEAEHSALNPLRAVWLTLTAAFLLTLLLQLLPPGLLPGCAIFQDLIRYGKTKCGEPSRPAACRAFDVPKRYFSHFYIISVL
WNGFLLWCLTQSLFLGAPFPSWLHGLLRILGAAQFQGGELALSAFLVLVFLWLHSLRRLFECLYVSVFSNVMIHVVQYCFGLVYY
VLVGLTVLSQVPMDGRNAYITGKNLLMQARWFHILGMMMFIWSSAHQYKCHVILGNLRKNKAGVVIHCNHRIPFGDWFEYVSSPN
YLAELMIYVSMAVTFGFHNLTWWLVVTNVFFNQALSAFLSHQFYKSKFVSYPKHRKAFLPFLF

>example_2 optional comment
ASQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPK
NMYKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGR
KSAHKGFKGVDAQGTLSKIFKLGGRDSRSGSPMARRELVISLIVES

 • Result (Stand format) :

>No.1 example_1
 • Half-life prediction result: Short-lived protein (Estimated half life: <30 minutes)
Items Content Ratio
Total amino acids : 318  
Proteinogenic amino acids : 318 100.0%
Additional amino acids (UO) : 0 0.0%
Ambiguous Amino Acids (BJZX) : 0 0.0%
Amino Acid A: 25 7.861%
Amino Acid C: 8 2.515%
Amino Acid D: 4 1.257%
Amino Acid E: 7 2.201%
Amino Acid F: 28 8.805%
Amino Acid G: 19 5.974%
Amino Acid H: 13 4.088%
Amino Acid I: 13 4.088%
Amino Acid K: 11 3.459%
Amino Acid L: 50 15.72%
Amino Acid M: 9 2.830%
Amino Acid N: 12 3.773%
Amino Acid P: 15 4.716%
Amino Acid Q: 11 3.459%
Amino Acid R: 13 4.088%
Amino Acid S: 20 6.289%
Amino Acid T: 10 3.144%
Amino Acid V: 25 7.861%
Amino Acid W: 11 3.459%
Amino Acid Y: 14 4.402%
     

>No.1 example_2 optional comment
 • Half-life prediction result: Non-Short-lived protein (Estimated half life: >=30 minutes)
Items Content Ratio
Total amino acids : 152 
Proteinogenic amino acids : 152100.0%
Additional amino acids (UO) : 00.0%
Ambiguous Amino Acids (BJZX) : 00.0%
Amino Acid A: 106.578%
Amino Acid D: 85.263%
Amino Acid E: 31.973%
Amino Acid F: 74.605%
Amino Acid G: 1711.18%
Amino Acid H: 106.578%
Amino Acid I: 63.947%
Amino Acid K: 127.894%
Amino Acid L: 85.263%
Amino Acid M: 31.973%
Amino Acid N: 31.973%
Amino Acid P: 117.236%
Amino Acid Q: 63.947%
Amino Acid R: 159.868%
Amino Acid S: 1610.52%
Amino Acid T: 85.263%
Amino Acid V: 63.947%
Amino Acid Y: 31.973%